青鳉鱼鳃内Na-\\'+--K-\\'+--ATPase基因的克隆及皮质醇对其表达的影响 - 图文  下载本文

参考文献431996,6:986-994.【38]WittwerDNACT,HerrmannMG,MossAA,eta1.Continuousfluorescencemonitoringofrapidcycleamplification[J】.Biotechniques,1997,22:130-138.TH,Pate/Bl(’CincoM,eta1.IdentificationofLeptospirabiflexabyreal-timehomogeneous【39]WoodetectionofrapidcyclePCRproduct【J】.JournalofMicrobiologicalMethods,1999,35:23-30.reverse【40lYinchainJL,ShackelNA,ZekryA,McGuninnessPH,eta1.Real—timeofcytokineandtranscriptase-lx)lymerasemRNAexpressionwithreaction(RT-PCR)formeasurementorgrowthfactorflourogenicprobeSYBRGreenI【J】.ImmunologyandCellBiology,2001,79:213—221【41]McCormickSD.EndocrinecontrolofosmotegulationinteleosttjshtJl.flu'neronZool,2001,41:781-794,【42]PelisRM,McCormickSD.EffectsofgrowthhormoneandcortisolNa+,K+,一CI—cotransporterlocalizationandabundanceinthegillsofAtlanticsalmon[J].GenCompEndocrinol,2001.124:134--143.【43]W.S.Marshall,S.E.Bryson.Transportmodelofamechanismsofseawaterteleostchloridecells:aninclusivemultifonctionalceu【J】.Comp.Biochem.Physi01.A,1998,119:97-106.a1.GeneexpressionofNa+一K+-ATPasealanda3subunitsingillsto[441s.H.Feng,J.H.Leu,C.H.Yang,etoftheteleost(Oreochromismossambicus)adapteddifferentenvironmentalsaimities,Mar[J].Bioteclmol,2002.4:379_-39l,【45]J.W.Semple,HJ.Green,P.M.Schulte.MolecularisoformsinFunduluscloningandcharacterizationoftwoNa/K-ATPaseheteroclitus[J].Mar.Biotechn01.2002.4:512-519.alpha【461J.G,Ricbards,J.W.Semple,J.S,Bystriansky,eta1.Na(+),l((+)-ATPasegillsofrainbowisoformswitchingintrout(Oncorhynchusmykiss)duringsalinitytransfer[J],J.Exp.Bi01.2003.206:4475-4486.andbeta[47]B.Blasiole,A.Degrave,V.Can.eld,eta1.DifferentialexpressionofNa,K-ATPasealphasubunitgenesinthedevelopingzebrafishinnerear[J].Dev.Dyn.,2003,228:386-392.cloningandcharacterizationof【48]J.W.Semple,HJ.Green,P.M.Schulte,MolecularisoformsinFundulustwoNa/K.ATPase‘heteroclitus,Mar.Bioteclmol,4(2002)512-519.Yan,】.H.Postlethwait,K.C.Cheng,R.Levenson,149]SJ.Rajarao,V.A.Can.eld,M.A.Mohideen,Y.LTherepertoireofNa,KATPasealphaandbetasubunitgenesexpressedinthezebrafishDaniorerio,GenomeRes.,11(2001)1211—1220.Geering,R,A,Farley,Thesodiumpumpneedsits[50]A.A.McDonough,k1990,4:1598-1605.b-subunit[J].FASEBJ.,[511温海深,高玲,李文阁.硬骨鱼类神经内分泌,免疫网络及其调控研究_中国海洋大学学报,2006,36(1):60~64【52]MareY,EngelsmaMO,HuisingWB,eta1.Neuroendocrineimmuneinteractionsinfish:aroleforinterlenkin21【Jj,Vet【53]Ainsworthlmmun01.,2002,87:467-479.AJ,DexiangC,WaterstratPR.ChangesinperipheralbloodleukocytepercentagesandAquatAnimHealth,1991,3:41-47.onfunctionofneutrophilsinstressedchannelcatfish[J】.J【54]EspelidS,LokkenGB,SteiroKeta1.EffectsofcortisolandstresstheimmunesysteminAtlanticSalmon(SalmosalarL.)【J1.Fish[55]AltschulSF,MaddenShellfishImmun01.,1996,6:95.110BLASTandPSIBLAST:AuewTL,SchafferAA,ZhangJ,eta1.Gappedgenerationofproteindatabasesearchprograms[J].NucleicAcidsResearch,1997,25:3389—3402【56】沈同,王镜岩.生物化学(下册).高等教育出版社[MI,1991,30.32[57]C.P.Cutler,I.L.Sanders,N.Hazon,ela1.Primarysequence,tissuespecificityandmRNAexpressionof竺the童塑鱼塑堕竖:兰二!!翌竺堇望塑塞堕垄堡堕壁翌茎室垄塑星堕Na++F-ATPaseB1subunitintheEuropeaneel(Anguillaanguilla)【J】FishPhysi01.Biochem,1995,14:423--429[58】sJ.Rajarao,VACaneld,M.A.Mohideen,eta1.nerepertoireofNa+.K+.ATPasealphaandbetasnbunitgenesexpressedinthezebrafish(Daniorerio)fJ】,GenomeRes.,2001,11:1211—1220expressionofNa,K-ATPasealphaandbeta【591B.Blasiole,A.Degrave,V.Can.eld,eta1.Differentialsubunitgenesinthedevelopingzebrafishinnereat【J】,Dev.Dyn.,2003,228:386-392【60]MauleA…GSchreckC…BStressandcortisoltreatmentchangedaffinityandnumberofglucocorticoidreceptorsinleukocytesandginofcohosalmon【J】.Gen.Comp.Endocrinol,1991,84:83-93.et(61]PriceS.R.,EnglandB.K,BaileyJ—La1.AcidosisandglucocorticoidsconcomitantlyincreaseubiquitinandproteasomesubunitmRNAsinratmuscle【J】.Am.J.Physi01.Cell.Physi01.1994,267,C9552960.【62]DucoretB.,TujagueM.,AshrafJ.,eta1.Cloningcontainsaofteleostfishglucocorticoidreceptorshowsthatitdeoxyribonucleicacid-bindingdomaindifferentfromthatofmammals[J].Endocrinology,1995,136,3774-3783,[63]MathilakathM.Vijayan,StavroulaglucocorticoidreceptorComparativeRaptis,RameshSathiyaa.Cortisoltreatmentaffectsandglucocorticoid-responsivegenesintheliverofrainbowtroutfJl.GeneralandEndocrinology,2003,132:256-263【64】阳成波,印遇龙,龚建华等.实时定量PeR研究进展及其应用[J】.中国预防兽医学报,20035(25):395—399[65]MadsenS.S..TheroleofcortisolandgrowthhormoneinseawateradaptationanddevelopmentofseahypoosmoregulatorymechanismsinEndocrin01.,1990b,79:1-11trout(parrSalmotruttatrutta)[JJGen.Comp.【66]JensenM.K.,MadsenS.S,,KristiansenK。OsmoregulationandsalinityeffectsseaontheexpressionandactivityofNa,K-ATPaseinthegillsofEuropean1998.282:29m.300bass(Dicentrarchuslabrax)[J].J.Exp.Z001.,(67]KellyS.P.,ChowI.N.k,WooN.Y…SHaloplasticityofblackseabream(Mfliomacrocephalusj:hypersalinetofreshwateracclimation【J】.J.Exp.Z001.,1999,283:226-241a1.Cortisolreceptorblockadeandseawateradaptationintheofexperimentalzoology,2005,303A:132—142【68]W.SMarshall,R.R.F.Cozzi,R.M.PelisetEuryhalinetelost(Fundulusheteroclitus)【J】.Journal【69]BuryteleostNR,SturmA,LeRouzicP,ela1.Evidencefortwodistinctfunctionalglucocorlicoidreceptorsinfish[J].JMolecEndocrinol,2003.31:141—156NR,ela1.Amineralocorticoid-likereceptorintherainbowtrout.[70]ColombeLFostierA,BuryOncorhynchusmykiss:CloningandcharacterizationofitssteroidbindingSteroids,2000.65:319—328domain[J].[71]CrockettEL.LipidrestructuringdoesnotcontributetoelevatedactivitiesofNa+/K+-ATPaseinbasolateralmembranesfromthegillofseawater‘acclimatedBi01.,1999,202:2385—2392eel(Anguillarostrata)【11.JExp【721DeaneEE,Woosarba)【J】.AmNYS—DifferentialgeneexpressionassociatedwitheuryhalinityinseabreamfSparusJPhysi01.,2004,287:R1054-R1063Butler[73]GreenwoodAKPC,WhiteRB,eta1.Multiplecorticoidreceptorsinateleostfish:Distinctsequences,expressionpatterns,andtranscriptionalactivities[J].Endocrinology,2003,144:4226-4236茎耋苎壁竺【74]HansenHJM,GrosellM..Aremembranelipidsinvolvedinosmoregulation?Studiesinvivoontheeuropeaneel,Anguillaanguilla,afterreducedambientsalinity[J].EnvBiolFishes,2004,70:57--65【75]SingerTD,ClementsKM,SempleJW,eta1.SeawatertoleranceandgeneexpressionintwostrainsofAtlanticsalmonsmolts[J].CanJFishAquatSci,2002,59:125—135【76]SingerTD,FinstadB,McCormickSD,eta1.InteractiveeffectsofcortisoltreatmentandambientseawaterchallengeongillNa+/K+-ATPaseandCFTRexpressionintwostrainsofAtlanticsalmonsmolts[J].Aquaculture,2003,222:15~28【77]SlomanKA,DesforgesPR,GilmourKM..Evidenceformineralocorticoid.1ikereceptorlinkedtobranchialchloridecellproliferationinfreshwaterrainbowtrout[J].JExpBiol,2001,204:3953-3961.【781KojiInoue,YoshioTakei.ReviewAsianmedakafishesoffernewmodelsforstudyingmechanismsofseawateradaptation[J1.ComparativeBiochemistryandPhysiologyPartB,2003,136:635“45【79]http://mbase.bioweb.ne.jp/【80]http://www.tigr.org/tdby/tgi/olgi/.【81]http://bioll.bio.nagoya-U.ac.jp:8000/ISMG’03C.html【82]MauleA.G.,SchreckC.B.,Glucocorticoidreceptorsinleukocytesandgillofiuvenilecohosalmon(Oncorynchuskisutch)[J1.Gen.Comp.Endocfinol,1990,77:44&-455[8310’MalleyB.W.,TsaiM。Molecularpathwaysofsteroidreceptoraction[J1.Bi01.Reprod,1992,46:163-167【84]PelisR.M.,ZydlewskiJ.,McCormick,S.D。GillNa+一K+-2CI.cotransporterabundanceandlocationinAtlanticsalmon:Effectsofseawaterandsmolting[J].Am.J.Physi01.,2001,280:R1844-R1852.’。[85]McCormickS.D。HormonalcontrolofgUlNa+.一-ATPaseandchloridecellfunction[J]FishPhysiology,1995,14:285-315【86]DangeA.D..BranchialNa+一K+.ATPaseactivityinfreshwaterorsaltwateracclimaledtilapia(Oreochrom如Sarotherodon)mossambicus:effectsofcortisolandthyroxine[J].Gen.Comp.Endocrin01.,1996,62:341-343【871LinL.,WengC.,HwangP—Effectsofcortisolandsalinitychallengeonwaterbalanceindevelopinglarvaeoftilapia(Oreochromismossambicus)【J1.Physinl.Biochem.Z001.,2000,73:283-289.【88]McCormick,s.D..Conisoldirectlystimulatesthedifferentiationofchloridecellintilapiaopercularmembrane[J].Am.J.Physi01.,1990,259:R857-R863.【89]MadsenS.S,,JensenM.k,KristiansenK,eta1.ExpressionofNa+-K+-ATPaseinthebrowntrout,Salmotrutta:invivomodulationbyhormonesandseawater【J】.Am.J.Physi01.,1995,269:R13339-R1345[90]SunnyF.,Oommen0.V~RapidactionofglucocorticiodsonbranchialATPaseactivityin(Oreochromismossambicus):aninvivoandinvitrostudy.Comp.Biochem.Physi01.,2001,B130:323-330英文名词及简写英汉名词及简写DegeneratePrimerDerivactive兼并引物荧光强度变化率焦碳酸二乙酯二甲基亚砜DiethylPyrocarbonate(DEPC)DimethylSulfoxide(DMSO)curvesDissociation熔解曲线Chemicals(EDCs)内分泌干扰物质基因表达糖皮质激素受体甘油醛3.磷酸脱氢酶皮质醇(又称氢化可的松)医药品和个人护理品成分EndocrineDisruptingGeneExpressionGlucocorticoidReceptor(GR)Glyceraldehyde??3??phosphatedehydrogenase(GAPDH)HydrocortisonePharmaceuticals&PersonalCareProductIngredients(PPCPs)PersistentOrganicPolychlorinatedPollutants(POPs)持久性有机污染物多氯联苯类催乳素实时定量RT-PCR序列比对a)钠钾ATP酶a亚基钠钾ATP酶B亚基Biphenyls(PCBs)Prolactin(PRURealTimeRT.PCRSequenceAlignmentSodiumpotassiumATPasealphasubunit(Na+一K+-ATPaseSodiumpotassiumATPasebetasubunit(Na+一K+一ATPaseB)致谢致谢本论文是在导师范光丽教授和胡建英教授的悉心指导下完成的。三年来,范老师渊博的学识、严谨求实的治学的作风、高尚的品德以及孜孜不倦的精神,使我在学习和研究中受益匪浅。范老师指导我进入动物解剖学这一学科,并让我深入的学习和实践,使我掌握了动物解剖学基础知识和研究技能。在范老师的支持和鼓励下,我才‘有机会去北京大学做毕业论文,开拓视野,接受新思想。在此向范老师表示最衷心的感谢和最崇高的敬意!师恩如海,无以为报,我只能将感激化作力量,在将来的学习和工作中加倍努力,报答恩师。北京大学胡建英教授为我进入北京大学完成硕士毕业论文研究提供了可贵的机会,从论文选题、实验设计、问题解决、结果讨论,到论文的最终完成,都倾注了胡老师大量的心血。胡老师渊博的学识,崇高的学术品质,敏锐的思维,严谨的科研精神使我受益终身,在此表示衷心感谢。感谢王建华教授、赵献军教授、陈树林副教授、卿素珠副教授、吉红副教授在实验开题上给予的指导。本论文是在北京大学环境学院完成的。特别感谢张照斌博士后,在我的实验过程中给予的大力支持和帮助。感谢金芬、安立会、安伟、常红、万神、金晓辉、刘金林、王乐征、镇华君、张振国、赛林霖等同学在学习和生活等方面给予我的帮助。我还真诚地感谢博士生乔海莲、王月影、周宏超、张永德、张艳、郑小敏和硕士生何玉龙、田光明、赵锦、许博、贾利云、曹力、许小成,以及已经毕业的陈正礼博士、王跃嗣博士和阿依木吉利硕士等在学习和生活等方面给予的许多关心和帮助。感谢我的父母,是他们为我的成长倾注了大量的心血,付出了艰辛的劳动,他们的支持与鼓励是我奋发向上的强大动力,是我能顺利完成学业的坚强后盾。再次感谢所有帮助我、关心我的师长、朋友和亲人。侯彦峰2006.6.6